Molecular Lab

What is DNA Sanger Sequencing?

Molecular Lab uses fluorescence-based cycle sequencing system which is an extension and refinement of Sanger dideoxy sequencing. we provides researchers speed, throughput and flexibility of DNA sequencing with the means to gain valuable insights at a rate never before possible with state of the art GENETIC ANALYZER SeqStudio by Thermo Fisher.

DNA sequencing applications and approaches

DNA sequencing can be used for many applications, including:

De novo sequencing of genomes
Detection of variants (SNPs) and mutations
Biological identification
Confirmation of clone constructs
Detection of methylation events
Gene expression studies
Detection of copy number variation.

STANDARD SEQUENCING SERVICE

The standard sequencing service provides customers fast
sequencing results of reading length up to 700 bp using
plasmid DNA or PCR product.

FULL-LENGTH SEQUENCING SERVICE (PRIMER WALKING)

This service is for sequence analysis of plasmid longer than 2 kb including a long insert or PCR product. The turnaround time is approximately one week for a 3 kb plasmid and can vary depending on a template length. Assembled analysis data will be delivered to customers along with an aligned file, single contig file as well as custom primers.

SEQUENCING SERVICE

Yield and purity of samples are measured.
Cyclic sequencing reaction using BigDye™ Terminator is performed.
The sequencing PCR product is puriﬁed.
Run sequencing reactions on ABI SeqStudio Genetic Analyzer.
Guaranteed length: 700 bp (based on a normal plasmid DNA).
Phred Score (QV): 20
Data ﬁle (ab 1, seq, .pdf, or .txt) and QC report are provided.
Complete analysis data is delivered to the customer.

FEATURES & BENEFITS

AL-RAZI LAB INTERNATIONAL DNA/RNA Sequencing Facility provides

Rapid Turnaround Time
Flexible Sample Submission I High Quality Results
Cost Effectiveness
Hassle Free Documentation

INTENDED FOR WHO!

The Sanger Sequencing service is intended for all customers in universities and research institutes. We are performing conventional Sanger Sequencing along with a range of service options to meet individual customer requirements.